Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes

Authors
[01]
Esra Maltas, Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey
[02]
Aslı Dageri, Department of Plant Protection, Faculty of Agriculture, Ankara U
[03]
Hasibe Cingilli Vural, Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey
[04]
Salih Yildiz, Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey

2d5cef432f41f7cb8a0f208a69b677ecAbstract
Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.

Keywords
Echinacea Purpurea and Echinacea Pallida, Molecular Marker, PCR, nDNA Antioxidant Activity, Fatty acid, Flavonoid

Full paper:http://bit.ly/1Kf9iwA

photo source:http://bit.ly/1Kpk2w2

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